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iqtree

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A transparent ONT bacterial assembly case study for an *Acinetobacter* barcode07 isolate, built not only to generate an assembly, but to show how raw reads become an interpretable genome through QC evidence, assembler comparison, graph checks, phylogeny, annotation, documented decisions, and honest limitations.

  • Updated Mar 26, 2026
  • HTML

This repository provides a comprehensive tutorial for phylogenetic analysis, covering data collection, sequence alignment, tree construction, and interpretation. It runs on AWS SageMaker using Jupyter notebooks and includes tools like MAFFT, Nextclade, and IQ-TREE.

  • Updated Jun 6, 2025
  • Jupyter Notebook

Automated maximum-likelihood phylogeny pipeline for viral families. Discovers species via NCBI Taxonomy, downloads from GenBank, aligns with MAFFT, builds trees with FastTree (broad) and IQ-TREE (refined), and annotates internal nodes by LCA. Supports multi-marker concatenation for large DNA virus families.

  • Updated Jul 9, 2026
  • Python

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