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TheThunda01/README.md

Brandon J. Huynh, MPH

Infectious Disease and Public Health Researcher exploring computational biology and mathematical modeling.

From biological measurements to better experiments

What can we trust in a measurement, what can a model explain, and what should we measure next?

My background spans chemistry, molecular laboratory science, epidemiology, and biostatistics. This portfolio connects those interests through public-data analyses, biological models, and tools for tracing evidence. The common thread is making the reasoning behind a result inspectable.

Start here

Three studies introduce the main question. They use different biological systems; this is a reading path, not a single analysis pipeline.

Study Question What to look for
1. Enzyme kinetics Can different explanations fit the same measurements? How the measured concentration range changes parameter uncertainty.
2. GFP policy comparison Does a more elaborate selection rule actually help? A recorded expectation, a matched comparison, and an unexpected result.
3. MAPK dynamics Which observation would distinguish competing explanations? A simulated cycle of choosing a measurement and updating model hypotheses.

Current investigation

The green fluorescent protein (GFP) comparison tests ways to select existing brightness measurements under a fixed budget. Across four seeds, diversity-only selection had the lowest mean error on more-distant variants; uncertainty-only selection had the lowest mean error on local variants. The combined policy did not support the prediction that it would help most at distance. These are exploratory results on reused public outcomes.

The next planned analysis asks whether the combined policy's shortlist excludes useful candidates. After that, the direction is to test when measurement selection fails in the mitogen-activated protein kinase (MAPK) simulation. The roadmap separates these proposals from completed work.

Explore the supporting work

  • A public-health connection: Surveillance data quality examines denominators and reporting windows before interpreting a trend.
  • A research workflow tool: EvidenceTrail makes extraction records and reviewer decisions inspectable. Try its browser demo with synthetic examples.
  • A visual introduction: Nature's Perfume Lab explores plant aroma chemistry and protein evidence through a familiar question.

The complete project guide places all fourteen projects in this story, including sequence and expression quality, model evaluation, structure and metabolism, and evidence-review planning.

Methods and transparency

These projects use attributed public data and labeled simulations; they do not report new laboratory experiments I conducted or establish new biological findings. They were developed with AI assistance. Tests check specified software behavior; scientific interpretation remains tied to the evidence and limitations in each project.

Project guide · Roadmap · Shared standards · Glossary · Sources · Maintenance and review history

Pinned Loading

  1. gfp-active-learning gfp-active-learning Public

    Retrospective active-learning comparisons on measured GFP fluorescence, with fixed test data, budgeted label acquisition, and explicit limits.

    Python

  2. mapk-dynamics-lab mapk-dynamics-lab Public

    A curated MAPK signaling model with reproducible dynamics, Bayesian inference, and virtual experiment selection.

    Python

  3. enzyme-kinetics-workbench enzyme-kinetics-workbench Public

    Mechanistic enzyme-rate fitting, identifiability diagnostics, and conditional experimental-design comparisons with public data.

    Python

  4. surveillance-data-quality surveillance-data-quality Public

    Real CDC data audits, denominator and window sensitivity, snapshot comparison, and synthetic as-of report histories in Python and SQLite.

    Python

  5. evidencetrail evidencetrail Public

    Checks extraction tables, compares reviewer records with explicit coverage and agreement denominators, and documents human review decisions.

    Python

  6. natures-perfume-lab natures-perfume-lab Public

    A reproducible case study connecting terpene chemistry, enzyme sequences, and structural evidence through an interactive molecular explorer.

    Python