@@ -0,0 +1,23 @@
+## Multimodal_breast_cancer_subtype_analysis
+Profiling intratumoural heterogeneity of breast cancer subtypes through integrated transcriptomic and metabolic analysis at single-cell and spatial resolution
+
+## Requirements
+### Install Pytorch and pytorch-geometric
+
+### Install POT: Python Optimal Transport
+```sh
+conda install -c conda-forge pot
+```
+### Install SpatialMST package for spatial multimodal integration
+PyPI package: https://pypi.org/project/SpatialMST
+```sh
+pip install SpatialMST
+pip install scanpy
+```
+
+### Generate metabolic flux rates and metabolite abundances for single-cell and spatial transcriptomics using scFEA.
+https://www.biorxiv.org/content/10.1101/2020.09.23.310656v1.full
+https://github.com/changwn/scFEA/tree/master
+
+## Download the breast cancer single_cell and spatial transcriptomics datasets from figshare
+[Dataset](https://figshare.com/articles/dataset/Datasets_for_breast_cancer_single-cell_and_spatial_transcriptomics_multimodal_integration_/30290779)